The Expression Disruption Screen, developed at José Luis Gómez-Skarmeta laboratory (GSlab), aims to detect and interfere with non coding cis-regulatory elements within the zebrafish genome. This is achieved by generating stable transgenic lines using a novel tool, the Expression Disruption (ED) vector:
ED is composed by two enhancer traps that allows to detect nearby enhancers, one of which has RFP and the other GFP as reporter genes. Also, ED contains a strong insulator that has the ability to block enhancers. The combination of these two tools allows to discriminate the regulatory information that is upstream and downstream of the insertion point by simply observing RFP and GFP expression. In addition, enhancers that are blocked regarding their endogenous promoters result in regulatory mutations.
The results of this screen are provided through this publicly available web browser in the hope to push further the exploration of the vertebrate non coding genome.
March 2014 - Genome resource: A mobile insulator system to detect and disrupt cis-regulatory landscapes in vertebrates.
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October 2013: ED Sequence page updated.
