Epigenome Browser


The generation of distinctive cell types that form different tissues and organs requires precise, temporal and spatial control of gene expression. This depends on specific cis-regulatory elements distributed in the non-coding DNA surrounding their target genes. Studies performed on mammalian embryonic stem cells and Drosophila embryos suggest that active enhancers form part of a defined chromatin landscape marked by histone H3 lysine 4 mono-methylation (H3K4me1) and histone H3 lysine 27 acetylation (H3K27ac). Nevertheless, little is known about the dynamics and the potential roles of these marks during vertebrate embryogenesis. We have provided genomic maps of H3K4me1/me3 and H3K27ac at four developmental time-points of zebrafish embryogenesis and analyze embryonic enhancer activity.


We have found that:

  • changes in H3K27ac enrichment at enhancers accompany the shift from pluripotency to tissue-specific gene expression;
  • in early embryos, the peaks of H3K27ac enrichment are bound by pluripotent factors such as Nanog;
  • the degree of evolutionary conservation is higher for enhancers that become marked by H3K27ac at the end of gastrulation suggesting their implication in the establishment of the most conserved (phylotypic) transcriptome that is known to occur later at the pharyngula stage.
CSIC1

Dynamics of enhancer chromatin signatures mark the transition from pluripotency to cell specification during embryogenesis.

O. Bogdanovic´, A. Fernández-Miñán, J. J. Tena, E. de la calle-Mustienes, C. Hidalgo, I. van Kruysbergen, S. J. van Heeringen, G. J. Veenstra and J. L. Gómez-Skarmeta.
Genome Res. 22, 2043-2053 (2012)
http://www.ncbi.nlm.nih.gov/pubmed/22593555
http://genome.cshlp.org/content/22/10/2043.abstract

The developmental epigenomics toolbox: ChIP-seq and MethylCap-seq profiling of early zebrafish embryos.

Ozren Bogdanovic´, Ana Fernández-Miñán, Juan J. Tena, Elisa de la Calle-Mustienes, José Luis Gómez-Skarmeta.
Methods, 2013-05-14
http://www.ncbi.nlm.nih.gov/pubmed/23624103
http://www.sciencedirect.com/science/article/pii/S1046202313001151

Our published data, together with public available RNA-seq, can be visualized at ZV9 public.

Our published data can be downloaded from:
   GEO H3K4me1 , H3K4me3 , H3K27ac , GEO H3K27me3.



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